platforms

The EVe Facility is a state-of-the-art academic research platform dedicated to supporting research groups, hospitals, and companies working in the field of extracellular vesicles (EVs). We offer cutting-edge technologies, expert know-how in sample preparation, and a full range of services for EV production, isolation, and characterization.

EV Isolation Services:

We provide access to multiple EV isolation techniques, tailored to your research needs:

  • Ultracentrifugation (UC, Optima XE90 – SW 32Ti, Beckmann Coulter)
  • Tangential Flow Filtration (TFF, KrosFlo® KR2i TFF System, Repligen)
  • Size Exclusion Chromatography (SEC)
  • Automated Nanofiltration (Exodus H-600, Proteingene – Exodus bio)

EV Characterization Services:

Our facility offers comprehensive EV characterization using advanced technologies:

  • Nanoparticle Tracking Analysis (NTA) (ZetaView, Particle Metrix)
  • Interferometric Light Microscopy (ILM) (Videodrop, Myriade)
  • Nano-Flow Cytometry (nFCM) (Nanoanalyzer, NanoFCM)
  • Super-Resolution Microscopy (Nanoimager, ONI)
 

We also provide complementary standard characterization methods such as:

  • Simple Western (Jess, Biotechne)
  • ELISA
  • Quantification of proteins, lipids, and nucleic acids

EV functional assay services:

Our facility offers various functional assays for testing EV functions (on demand).

Consultation Services:

Our experts are available to guide you in designing workflows for EV separation, characterization, and downstream analysis.

What we offer:

  • Access to Equipment
    We provide personalized training to help users gain full autonomy on our instruments. Once trained, users can independently book and operate the equipment, allowing greater flexibility and efficiency in their research.
  • Service-Based Analyses
    Prefer to delegate the work? Our team can perform the experiments and data acquisition for you, ensuring high-quality and reliable results.
  • Collaborative Projects

Pricing informations:

For pricing or a personalized quote, please contact us directly (marie.maumus[a]inserm.fr or daniele.noel[a]inserm.fr). We’re happy to assist you in planning your project.

Partner with the EVe Core Facility to accelerate your EV research with precision, innovation, and reliability.

ScientifiC LEADER

Danièle Noël

daniele.noel[a]inserm.fr

Technical manager

Marie Maumus

marie.maumus[a]inserm.fr

Key publications

Original publications:

− Anti-aging effect of extracellular vesicles from mesenchymal stromal cells on senescence-induced chondrocytes in osteoarthritis. Boulestreau J, et al. Aging (Albany NY). 2024 Nov 22;16(21):13252-13270

− Extracellular vesicles from senescent mesenchymal stromal cells are defective and cannot prevent osteoarthritis. Boulestreau J, et al. J Nanobiotechnology. 2024 May 16;22(1):255.

− Lung Fibrosis Is Improved by Extracellular Vesicles from IFNγ-Primed Mesenchymal Stromal Cells in Murine Systemic Sclerosis. Rozier P, et al. Cells. 2021 Oct 13;10(10):2727.

− Extracellular Vesicles Are More Potent Than Adipose Mesenchymal Stromal Cells to Exert an Anti-Fibrotic Effect in an In Vitro Model of Systemic Sclerosis. Rozier P, et al. Int J Mol Sci. 2021 Jun 25;22(13):6837.

− Mesenchymal stromal cells-derived extracellular vesicles alleviate systemic sclerosis via miR-29a-3p. Rozier P, et al. J Autoimmun. 2021 Jul;121:102660.

− TGFBI secreted by mesenchymal stromal cells ameliorates osteoarthritis and is detected in extracellular vesicles. Ruiz M, et al. Biomaterials. 2020 Jan;226:119544.

− Mesenchymal stem cells-derived exosomes are more immunosuppressive than microparticles in inflammatory arthritis. Cosenza S, et al. Theranostics. 2018 Feb 3;8(5):1399-1410.

− Mesenchymal stem cells derived exosomes and microparticles protect cartilage and bone from degradation in osteoarthritis. Cosenza S, et al. Sci Rep. 2017 Nov 24;7(1):16214.

Reviews:

− Therapeutic potential of extracellular vesicles derived from mesenchymal stromal cells in rheumatic diseases. Bertolino GM, et al. Nat Rev Rheumatol. 2024 Jun;20(6):389.

− Recent Advances in Extracellular Vesicle-Based Therapies Using Induced Pluripotent Stem Cell-Derived Mesenchymal Stromal Cells. Bertolino GM, et al. Biomedicines. 2022 Sep 14;10(9):2281.

− Development of extracellular vesicle-based medicinal products: A position paper of the group “Extracellular Vesicle translatiOn to clinicaL perspectiVEs – EVOLVE France”. Silva AKA, et al. Adv Drug Deliv Rev. 2021 Dec;179:114001.

− Extracellular vesicles from mesenchymal stromal cells: Therapeutic perspectives for targeting senescence in osteoarthritis. Boulestreau J, et al. Adv Drug Deliv Rev. 2021 Aug;175:113836.

− Mesenchymal Stromal Cell-Derived Extracellular Vesicles Regulate the Mitochondrial Metabolism via Transfer of miRNAs. Loussouarn C, et al. Front Immunol. 2021 Mar 16;12:623973.

− Mesenchymal Stem Cell-Derived Extracellular Vesicles: Opportunities and Challenges for Clinical Translation. Maumus M, et al. Front Bioeng Biotechnol. 2020 Sep 10;8:997.

− Mesenchymal Stem Cell Derived Extracellular Vesicles in Aging. Boulestreau J, et al. Front Cell Dev Biol. 2020 Feb 21;8:107.

− Pathogenic or Therapeutic Extracellular Vesicles in Rheumatic Diseases: Role of Mesenchymal Stem Cell-Derived Vesicles. Cosenza S, et al. Int J Mol Sci. 2017 Apr 22;18(4):889.

− Mesenchymal stem cells in regenerative medicine applied to rheumatic diseases: role of secretome and exosomes. Maumus M, et al.         Biochimie. 2013 Dec;95(12):2229-34.