Genome and Stem Cell Plasticity in Development and Ageing
Jean-Marc LEMAITRE
Responsable
Coordonnées :
Email : jean-marc.lemaitre@inserm.fr
Le groupe :
LEMAITRE Jean-Marc (DR1/Inserm)
Mots-clés
aging
epigenetics
senescence
chromatin
replicative stress
rejuvenation
regeneration
pluripotency
iPSC reprogramming
disease modeling
Techniques utilisées - Savoir faire
Culture cell : Cell lines, primary fibroblates and keratinocytes, Neural stem cells (NSC), Hematopoietic stem cell (HSC), Mesenchymal stem cells (MSC), pluripotent stem cells (ESC and iPSC)
Gene expression : Transcriptomics, RNA seq
Epigenetics: CHiP seq, HI-C
Replication studies: Replication timing analysis genome-wide, Origin mapping SNS purification.
Bioinformatics
Reprogramming strategies iPSC
Diffrentiation protocols into fibroblates, keratinocytes, NSC, HSC, MSC, cardiomyocytes
Mice models
en construction…
Références bibliographiques
– Lemey C et al. iPSCs as a major opportunity to understand and cure age-related diseases. Biogerontology 2015 published online 17may DOI 10.1007/s10522-015-9579-7
– Rondinelli B et al. H3K4me3 demethylation by the histone demethylase KDM5C/JARID1C promotes DNA replication origin firing. Nucleic Acids Res. 2015 Mar 11;43(5):2560-74.
– Bai Q et al. Temporal analysis of genome alterations induced by single-cell passaging in human embryonic stem cells. Stem Cells Dev. 2015 Mar 1;24(5):653-62.
– Lemaitre JM. Réversibilité du destin cellulaire : hasard ou nécessité biologique ? Biofutur 2014 nov n° 359 p31-33.
– Besnard E et al. Best Practices for Mapping Replication Origins in Eukaryotic Chromosomes. Current Protocols in Cell Biology 22.18.1-22.18.13, 2014 sept Published online in Wiley Online Library.
– Desprat R et al. The Molecular Machinery of Somatic Cell Reprogramming. Stem Cell Biology and Regenerative Medicine Book. River Publishers 2014 Editors P. Chabord and C ; Durand,. 10 (11) ISBN 978-87-93237-07-0(Print) 978-87-93237-08-7(Ebook)
– Milhavet O and Lemaitre JM. Senescence-derived pluripotent stem cells are able to re differentiate into fully rejuvenated cells. Tumor dormancy and cellular quiescence and senescence. Springer Sciences Editor. Hayat Ed., Book Chapter 2014 Volume 2: Aging, Cancer p265-276
– Venables JP et al. MBNL1 and RBFOX2 cooperate to establish a splicing program involved in pluripotent stem cell differentiation. Nat Commun. 2013;4:2480.
– Bai, Q et al. Embryonic Stem Cells Or Induced Pluripotent Stem Cells? A DNA Integrity Perspective. Curr Gene Ther. 2013 Apr;13(2):93-8.
– Bonne-Andrea C et al. SUMO2/3 modification of Cyclin E contributes to the control of replication origin firing. Nat Commun. 2013 ;4:1850.
– Besnard E et al. Unraveling cell-type specific and reprogrammable human replication origin signatures associated to G-quadruplex consensus motifs. Nature Struct & Mol Biol 2012 Jul 1. Doi :10.1038/nsmb.2339.
– Lapasset L et al. Rejuvenating senescent and centenarian human cells by reprogramming through the pluripotent state. Genes Dev, 2011 Nov;25(21):2248-53.
– Prieur A et al. p53/p21CIP1 and p16INK4A independent induction of senescence by a Chromatin dependent alteration of S- phase progression in absence of DNA damage. Nat Comms. 2011 13 Sept. 2:473. DOI: 10.1038/ncomms1473 .
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